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Cryo-EM-GLACIOS

Cryo-EM-GLACIOS

Who We Are

The IKI Cryo Electron Microscopy Unit provides expertise and resources for solving macro-molecular structures. Structure determination is enabled by a high-end transmission electron microscope (TEM), (FEI Glacios). The Thermo Fisher Scientific Glacios is a 200 kV transmission electron microscope with X-FEG and Falcon 4i detector coupled to a Selectris X energy filter for high-resolution structure determination of biological (and other soft matters) macromolecules. Its 12-grid autoloader allows efficient loading and unloading of CryoEM grids. The automated EPU and Tomo software systems are used for automated data collection, capable of producing more than 300 movies per hour, for high-resolution single particle analysis and cryo-electron tomography.

Support in Short

Our CryoEM unit, located at BGU Marcus campus is equipped with all the cutting-edge technology required to determine the three-dimensional CryoEM structures of biological macromolecules. Users will have full access to our Cryo-TEM (200 kV Thermo-Fisher Glacios, equipped with a Selectis X energy filter and Falcon 4i direct electron detector), as well as all required sample-preparation equipment and the computational support necessary for single-particle analysis, Cryo-Electron tomography, and cryo-electron diffraction.